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Hitrap capto q11/26/2022 ![]() marxianus is a good host for the production of dengue virus NS1 protein, which has potential for diagnostic applications. Protease activity was not detected in post-growth medium. Protein production was investigated under two conditions: with galactose and biotin pulses at 24-h intervals during 96 h of induction and without galactose and biotin supplementation. After induction with galactose, the NS1 protein was analyzed by sodium dodecyl sulfate-PAGE and immunogenic detection. Analysis of recombinant strains by RT-PCR and protein detection using blot analysis confirmed both transcription and expression of extracellular NS1 polypeptide. The transformed cells, selected in yeast extract peptone dextrose containing 200 μg mL −1 Geneticin, were mitotically stable. lactis α-mating factor signal peptide, and the recombinant plasmid obtained was used to transform K. The synthetic gene was cloned in frame with K. The NS1 gene sequence was codon-optimized to increase the level of protein expression in yeast. marxianus UFV-3 genome at the LAC4 locus using an adapted integrative vector designed for high-level expression of recombinant protein in Kluyveromyces lactis. The dengue virus type 1 nonstructural protein 1 (NS1) was integrated into the K. Here, we report expression of a dengue virus protein in K. However, despite its potential, there are few studies reporting the expression of heterologous proteins using this yeast. The yeast Kluyveromyces marxianus is a convenient host for industrial synthesis of biomolecules. ![]()
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